I-001

Maria Jesús Lobo Castañón

mjlc@uniovi.es

Ana Díaz-Fernández, Miguel Aller-Pellitero, Natalia Díaz, Dimas Suárez, Noemí de-los-Santos-Álvarez

Department of Physical and Analytical Chemistry. Universidad de Oviedo. Av. Julián Clavería, 8, 33006 Oviedo, Spain and Health Research Institute of Principado de Asturias, Av. Roma s/n, 33012, Oviedo, Spain

Advancing health monitoring with electrochemical aptamer sensors: a path to non-invasive molecular diagnostics

Recent advances in electrochemical aptamer-based sensors are pioneering new frontiers in precision medicine, enabling non-invasive, real-time, and personalized health monitoring. These sensors combine the high specificity and affinity of aptamers for target biomolecules with the sensitivity of electrochemical transduction, offering high-performance sensing in formats optimized for real-world applications. Their ability to address a broad spectrum of medical challenges has fueled a rapidly expanding research field. In this work, we present the design and development of electrochemical aptamer-based sensors for various healthcare applications. The sensing strategies that underpin these technologies are first introduced, followed by a discussion of key factors influencing their optimal use, including aspects such as aptamer selection, post-SELEX optimization, sensor fabrication, and analytical performance characterization. Finally, we provide a comprehensive analysis of the critical challenges in translating these technologies into clinical and consumer settings, highlighting potential solutions and future directions.

Acknowledgements: The work has been financially supported by Spanish Government (project PID2021-123183OB-I00), and Principado de Asturias (IDE/2024/000677).